Epitope‐based peptide vaccine design and target site depiction against Middle East Respiratory Syndrome Coronavirus: an immune-informatics study
Identifieur interne : 000660 ( Main/Exploration ); précédent : 000659; suivant : 000661Epitope‐based peptide vaccine design and target site depiction against Middle East Respiratory Syndrome Coronavirus: an immune-informatics study
Auteurs : Muhammad Tahir Ul Qamar [République populaire de Chine] ; Saman Saleem [Pakistan] ; Usman Ali Ashfaq [Pakistan] ; Amna Bari [République populaire de Chine] ; Farooq Anwar [Pakistan] ; Safar Alqahtani [Arabie saoudite]Source :
- Journal of Translational Medicine [ 1479-5876 ] ; 2019.
Abstract
Middle East Respiratory Syndrome Coronavirus (MERS-COV) is the main cause of lung and kidney infections in developing countries such as Saudi Arabia and South Korea. This infectious single-stranded, positive (+) sense RNA virus enters the host by binding to dipeptidyl-peptide receptors. Since no vaccine is yet available for MERS-COV, rapid case identification, isolation, and infection prevention strategies must be used to combat the spreading of MERS-COV infection. Additionally, there is a desperate need for vaccines and antiviral strategies.
The present study used immuno-informatics and computational approaches to identify conserved B- and T cell epitopes for the MERS-COV spike (S) protein that may perform a significant role in eliciting the resistance response to MERS-COV infection.
Many conserved cytotoxic T-lymphocyte epitopes and discontinuous and linear B-cell epitopes were predicted for the MERS-COV S protein, and their antigenicity and interactions with the human leukocyte antigen (HLA) B7 allele were estimated. Among B-cell epitopes, QLQMGFGITVQYGT displayed the highest antigenicity-score, and was immensely immunogenic. Among T-cell epitopes, MHC class-I peptide YKLQPLTFL and MHC class-II peptide YCILEPRSG were identified as highly antigenic. Furthermore, docking analyses revealed that the predicted peptides engaged in strong bonding with the HLA-B7 allele.
The present study identified several MERS-COV S protein epitopes that are conserved among various isolates from different countries. The putative antigenic epitopes may prove effective as novel vaccines for eradication and combating of MERS-COV infection.
Url:
DOI: 10.1186/s12967-019-2116-8
PubMed: 31703698
PubMed Central: 6839065
Affiliations:
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populaire de Chine</country><wicri:regionArea>Wuhan</wicri:regionArea></affiliation></author><author><name sortKey="Saleem, Saman" sort="Saleem, Saman" uniqKey="Saleem S" first="Saman" last="Saleem">Saman Saleem</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.411786.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0637 891X</institution-id><institution>Department of Bioinformatics and Biotechnology,</institution><institution>Government College University Faisalabad,</institution></institution-wrap>Faisalabad, Pakistan</nlm:aff><country xml:lang="fr">Pakistan</country><wicri:regionArea>Faisalabad</wicri:regionArea></affiliation></author><author><name sortKey="Ashfaq, Usman Ali" sort="Ashfaq, Usman Ali" uniqKey="Ashfaq U" first="Usman Ali" last="Ashfaq">Usman Ali Ashfaq</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id 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Chine</country><wicri:regionArea>Wuhan</wicri:regionArea></affiliation></author><author><name sortKey="Anwar, Farooq" sort="Anwar, Farooq" uniqKey="Anwar F" first="Farooq" last="Anwar">Farooq Anwar</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.412782.a</institution-id><institution-id institution-id-type="ISNI">0000 0004 0609 4693</institution-id><institution>Department of Chemistry,</institution><institution>University of Sargodha,</institution></institution-wrap>Sargodha, Pakistan</nlm:aff><country xml:lang="fr">Pakistan</country><wicri:regionArea>Sargodha</wicri:regionArea></affiliation></author><author><name sortKey="Alqahtani, Safar" sort="Alqahtani, Safar" uniqKey="Alqahtani S" first="Safar" last="Alqahtani">Safar Alqahtani</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdul Aziz University, Alkharj, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdul Aziz University, Alkharj</wicri:regionArea><wicri:noRegion>Alkharj</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Translational Medicine</title><idno type="eISSN">1479-5876</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Middle East Respiratory Syndrome Coronavirus (MERS-COV) is the main cause of lung and kidney infections in developing countries such as Saudi Arabia and South Korea. This infectious single-stranded, positive (+) sense RNA virus enters the host by binding to dipeptidyl-peptide receptors. Since no vaccine is yet available for MERS-COV, rapid case identification, isolation, and infection prevention strategies must be used to combat the spreading of MERS-COV infection. Additionally, there is a desperate need for vaccines and antiviral strategies.</p></sec><sec><title>Methods</title><p id="Par2">The present study used immuno-informatics and computational approaches to identify conserved B- and T cell epitopes for the MERS-COV spike (S) protein that may perform a significant role in eliciting the resistance response to MERS-COV infection.</p></sec><sec><title>Results</title><p id="Par3">Many conserved cytotoxic T-lymphocyte epitopes and discontinuous and linear B-cell epitopes were predicted for the MERS-COV S protein, and their antigenicity and interactions with the human leukocyte antigen (HLA) B7 allele were estimated. Among B-cell epitopes, QLQMGFGITVQYGT displayed the highest antigenicity-score, and was immensely immunogenic. Among T-cell epitopes, MHC class-I peptide YKLQPLTFL and MHC class-II peptide YCILEPRSG were identified as highly antigenic. Furthermore, docking analyses revealed that the predicted peptides engaged in strong bonding with the HLA-B7 allele.</p></sec><sec><title>Conclusion</title><p id="Par4">The present study identified several MERS-COV S protein epitopes that are conserved among various isolates from different countries. 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sortKey="Teng, Y" uniqKey="Teng Y">Y Teng</name></author><author><name sortKey="Wu, M" uniqKey="Wu M">M Wu</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Hu, N" uniqKey="Hu N">N Hu</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li><li>Pakistan</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Tahir Ul Qamar, Muhammad" sort="Tahir Ul Qamar, Muhammad" uniqKey="Tahir Ul Qamar M" first="Muhammad" last="Tahir Ul Qamar">Muhammad Tahir Ul Qamar</name></noRegion><name sortKey="Bari, Amna" sort="Bari, Amna" uniqKey="Bari A" first="Amna" last="Bari">Amna Bari</name></country><country name="Pakistan"><noRegion><name sortKey="Saleem, Saman" sort="Saleem, Saman" uniqKey="Saleem S" first="Saman" last="Saleem">Saman Saleem</name></noRegion><name sortKey="Anwar, Farooq" sort="Anwar, Farooq" uniqKey="Anwar F" first="Farooq" last="Anwar">Farooq Anwar</name><name sortKey="Ashfaq, Usman Ali" sort="Ashfaq, Usman Ali" uniqKey="Ashfaq U" first="Usman Ali" last="Ashfaq">Usman Ali Ashfaq</name></country><country name="Arabie saoudite"><noRegion><name sortKey="Alqahtani, Safar" sort="Alqahtani, Safar" uniqKey="Alqahtani S" first="Safar" last="Alqahtani">Safar Alqahtani</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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